Collection tubes 2 ml 50 250 loading dye 110 l 550 l handbook 1 1 minelutegel extraction kits 50 250 catalog no. For dna cleanup from enzymatic reactions using this protocol, add 3. The maximum amount of gel per spin column is 400 mg. For gel extractioncleanup of up to 10 micro g dna 70 bp to 10 kb from enzymatic reactions. The qiaquick gel extraction kit provides spin columns, buffers, and collection tubes for silicamembranebased purification of dna fragments from gels up to 400 mg slices or enzymatic reactions. Qiagen qiaquick gel extraction kit, 250 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extraction cleanup of up to 10. Qiaquick gel extraction kit protocol using a microcentrifuge chen. Qiaquick pcr purification kit 250, 28106 from qiagen labsave.
Apgx4 apgx50 apgx 250 kit size 4 preps 50 preps 250 preps miniprep column 4 50 250 2 ml microfuge tube 4 50 250 1. Gel extraction protocol qiaquick gel extraction kit protocol. Qiagen qiaquick pcr purification kit, 250 rxns, 10g binding capacity, 30l elution volume, tube format, manual processing, silica technology, 100 bp to 10 kb fragment, qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. The purelink quick gel extraction kit allows you to rapidly and efficiently purify dna fragments from tae or tbe agarose gels of various percentages. For purification of up to 10 micro g pcr products, 100 bp to 10 kb. Qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. Why is the concentration of dna very low after a gel extraction. Qiaquick gel extraction kit protocol syracuse university. Dna can be extracted and purified from agarose gels with different melting points in 30 minutes using purelink silica membranebased quick gel extrac. The innovative qiacube uses advanced technology to. This kit is specially designed for fast and easy isolation of smaller dna fragments 70 bp 4 kb from pcr reactions, agarose gels, or enzymatic reactions with an elution volume of only 10 l. Qiagen qiaquick gel extraction kit, 250 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extractioncleanup of up to 10micro g. This kit can also be used to purify dna from polyacrylamide gels. Plasmid dna purification genomic dna purification cat.
Qiaquick gel extraction kit 250 laboratory consumables. For dna fragments 8 kb, following the incubation step, add one additional volume equal to that of the gel slice of. This protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. Genelute gel extraction kit 70 purifications sigmaaldrich. Qiavac vacuum manifolds 30 handling guidelines for qiavac 6 31 qiavac 24 32 ordering information 33 qiagen companies and distributors 35 qiaquick spin handbook 072002 3. If you are viewing this page as a nonregistered user, the prices displayed is list price. Gel extraction protocol qiaquick gel extraction kit. I put in 1ug and i am getting back about 10nul but, my 260280s are jumping all over the place, as is my concentrations. Up to 400 mg agarose can be processed per spin column.
Add 3 volumes of buffer qg to 1 volume of gel i used the back of 200ul tips for gel excision and assumed the weight was about 150mg and therefore used 450ul of buffer qg 3. Qiaquick pcr purification kit 250 from qiagen sample to. Qiaquick nucleotide removal kit protocol 21 qiaquick gel extraction kit protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 troubleshooting guide 28 appendix. Qiaquick gel extraction kit 250 from qiagen selectscience. For departmental related matters, please visit the department of molecular, cell, and developmental biology website. Add 3 volumes of adb to each volume of agarose excised from the gel e. The genelute gel extraction kit is designed for the rapid purification of linear and plasmid dna fragments from standard or lowmelting agarose gels. Incubate at 50c for 10 min or until the gel slice has completely dissolved. From solutions from gels qiaquick qiaquick pcr nucleotide qiaquick gel qiaquick gel purification kit removal kit extraction kit extraction kit alkaline phosphatase yes yes yes yes cdna synthesis yes no no yes. Qiaquick pcr purification kit protocols 18 using a microcentrifuge 18 using a vacuum manifold 19 qiaquick nucleotide removal kit protocol 21 qiaquick gel extraction kit protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 troubleshooting guide 28 appendix. For purification of up to 10 g pcr products, 100 bp to 10 kb. Incubate at 3755 c for 510 minutes until the gel slice is completely dissolved2.
Automated dna cleanup the qiaquick pcr purification kit and qiaquick gel extraction kit can be fully automated on the. Recoveries of dna fragments in the size range between removed and recovered are not defined. These are available online in convenient and compact pdf format at. Add 3 volumes buffer qg to 1 volume gel 100 mg gel 100. I used the qiaquick gel extraction kit and followed the protocol supplied with the kit. Can qiaquick gel extraction kit purified dna be used for. This kit can also be used for dna cleanup from enzymatic reactions see page 8. The innovative qiacube uses advanced technology to process qiagen spin columns, enabling seamless integration of automated, lowthroughput sample prep into your laboratory workflow. Using a microcentrifuge or vacuum manifold, dna ranging from 70 bp to 10 kb is purified from 124 samples. Qiagen qiaquick pcr purification kit, 250 rxns, 10g binding capacity, 30l elution volume, tube format, manual processing, silica technology, 100 bp to 10 kb fragment, 40mers fragments removed, ideal for sequencing, microarray analysis, ligation and transformation, restriction. Qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. Banerjee lab ucla molecular, cell and developmental biology.
Qiavac vacuum manifolds 30 handling guidelines for qiavac 24 31 qiavac 6 32. Jenelle meyer 62007 university of wisconsinmadison. I always carry out the gel extraction optional steps and they have always gone fine. This products resides on a fisher scientific gsa or va contract. Qiagen qiaquick pcr purification kit, 250 rxns, 10g binding capacity, 30l elution volume, tube format, manual processing, silica technology, 100 bp to 10 kb fragment. Kit contents qiaquick pcr purification kits 50 250 catalog no. Regardless of 260230 or 260280, it is always optimal to get at least 1. A260230 readings post gel extraction general lab techniques. The minelute pcr purification kit and minelute gel extraction kit can be fully automated on the qiacube.
Qiagen qiaquick gel extraction kit, 250 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extractioncleanup of up to 10. Jul 22, 2009 i use the qiagen gel extraction kit all the time and have never encountered such a low 260230. Poor yield with gel extraction kit for about the umpteenth time now, i have gotten poor yield isolating my cut fragment from a 1% agarose gel. These are available online in convenient and compact pdf format. Qiaquick pcr purification kit 250 from qiagen selectscience. Qiaquick gel extraction kit the qiaquick gel extraction kit cat. Brand thermo scientific qiagen machereynagel promega invitrogen kit and no. Gel extraction protocol qiaquick gel extraction kit protocol 1.
This protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. For cleanup of other enzymatic reactions, follow the protocol as described for pcr samples or use the minelute reaction cleanup kit. The qiaquick gel extraction kit enables removal of nucleotides, enzymes, salts, agarose, ethidium bromide, and other impurities from samples, ensuring up to 80% recovery of dna see figure high recoveries from gels. Axyprep dna gel extraction kit for the rapid purification of dna fragments from agarose gels kit contents, storage and stability cat. Invitrogen purelink pcr purification kit 250 preps. Related products include the qiaquick nucleotide removal kit and qiaquick gel extraction kit. Dna ranging from 70 bp to 10 kb is purified using a simple and fast bindwashelute procedure and an elution volume of 3050. Qiaquick gel extraction kit protocol using a microcentrifuge. Qiaquick gel extraction kit protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 troubleshooting guide 28 appendix. Qiaquick gel extraction kit 250, 28706 from qiagen labsave. Qiaquick gel extraction kit b 0 4 p b 0 4 fragments removed fragments recovered figure 1.
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